Stoilov et al. Karyotype reconstruction modulates the sensitivity of barley genome to radiation induced DNA and chromosomal damage

ImageThe Bulgarian radiation biology, pioneers in application of COMET assays for radiation damage of DNA in plants, suggest that a barley line with multiple reciprocal translocations has high sensitivity to DNA strand breaks with less ability to maintain genome integrity.

intranet: BarleyRadSensitivitymutage_ges065.pdf

Karyotype reconstruction modulates the sensitivity of barley genome to radiation induced DNA and chromosomal damage Lubomir Stoilov, Mariyana Georgieva, Vasilissa Manova, Luxiang Liu, Kostadin Gecheff Institute of Plant Physiology and Genetics, Department of Molecular Genetics, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria

Mutagenesis pp. 1–8, 2012 doi: <a href=”http://dx.doi.org/10.1093/mutage/ges065&#8243; target=”_blank”>dx.doi.org/10.1093/mutage/ges065</a>

Email: molgen @ bas.bg The potential of cytologically reconstructed barley line D-2946 to cope with the major lesions that hamper genome integrity,<br />namely DNA single- and double-strand breaks was investigated.<br />Strand breaks induced by γ-rays and Li ions were<br />assessed by neutral and alkaline comet assay. Repair capacity<br />after bleomycin treatment was evaluated by agarose gel electrophoresis<br />under neutral and alkaline conditions. Frequencies<br />of radiation-induced chromosome aberrations were also<br />determined. Results indicate that radiation-mediated constitutive<br />rearrangement of the chromosome complement has led<br />to a substantial modulation of the sensitivity of barley genome<br />towards DNA strand breaks, produced by ionising radiation,<br />Li ion implantation and bleomycin in an agent-specific manner,<br />as well as of the clastogenic response to γ-rays. Based on<br />these findings, reconstructed barley karyotype D-2946 can be<br />considered a candidate radio-sensitive line with reduced ability to maintain genome integrity with respect to both DNA and chromosomal damage.

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